TY  - JOUR
A1  - Diekmann, Robin
A1  - Wolfson, Deanna L.
A1  - Spahn, Christoph Klaus
A1  - Heilemann, Mike
A1  - Schüttpelz, Mark
A1  - Huser, Thomas
T1  - Nanoscopy of bacterial cells immobilized by holographic optical tweezers
T2  - Nature Communications
N2  - Imaging non-adherent cells by super-resolution far-field fluorescence microscopy is currently not possible because of their rapid movement while in suspension. Holographic optical tweezers (HOTs) enable the ability to freely control the number and position of optical traps, thus facilitating the unrestricted manipulation of cells in a volume around the focal plane. Here we show that immobilizing non-adherent cells by optical tweezers is sufficient to achieve optical resolution well below the diffraction limit using localization microscopy. Individual cells can be oriented arbitrarily but preferably either horizontally or vertically relative to the microscope’s image plane, enabling access to sample sections that are impossible to achieve with conventional sample preparation and immobilization. This opens up new opportunities to super-resolve the nanoscale organization of chromosomal DNA in individual bacterial cells.
KW  - Bacterial genomics
KW  - Nanoscale biophysics
KW  - Optical tweezers
KW  - Super-resolution microscopy
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/47842
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-478427
SN  - 2041-1723
N1  - Rights and permissions: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
VL  - 7
IS  - Art. 13711
SP  - 1
EP  - 7
PB  - Nature Publishing Group UK
CY  - [London]
ER  -