TY  - JOUR
A1  - Kaur, Harmandeep
A1  - Carvalho, Jorge
A1  - Looso, Mario
A1  - Singh, Pratibha
A1  - Chennupati, Ramesh
A1  - Preussner, Jens
A1  - Günther, Stefan
A1  - Albarrán Juárez, Julián Alberto
A1  - Tischner, Denise
A1  - Classen, Simon
A1  - Offermanns, Stefan
A1  - Wettschureck, Nina
T1  - Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system
T2  - Nature Communications
N2  - G-protein-coupled receptor (GPCR) expression is extensively studied in bulk cDNA, but heterogeneity and functional patterning of GPCR expression in individual vascular cells is poorly understood. Here, we perform a microfluidic-based single-cell GPCR expression analysis in primary smooth muscle cells (SMC) and endothelial cells (EC). GPCR expression is highly heterogeneous in all cell types, which is confirmed in reporter mice, on the protein level and in human cells. Inflammatory activation in murine models of sepsis or atherosclerosis results in characteristic changes in the GPCR repertoire, and we identify functionally relevant subgroups of cells that are characterized by specific GPCR patterns. We further show that dedifferentiating SMC upregulate GPCRs such as Gpr39, Gprc5b, Gprc5c or Gpr124, and that selective targeting of Gprc5b modulates their differentiation state. Taken together, single-cell profiling identifies receptors expressed on pathologically relevant subpopulations and provides a basis for the development of new therapeutic strategies in vascular diseases.
KW  - Atherosclerosis
KW  - Experimental models of disease
KW  - High-throughput screening
KW  - Reverse transcription polymerase chain reaction
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/48427
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-484276
SN  - 2041-1723
N1  - Rights and permissions: Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
VL  - 8
IS  - Art. 15700
SP  - 1
EP  - 15
PB  - Nature Publishing Group UK
CY  - [London]
ER  -