TY  - JOUR
A1  - Braner, Markus
A1  - Koller, Nicole
A1  - Knauer, Julia
A1  - Herbring, Valentina
A1  - Hank, Susanne
A1  - Wieneke, Ralph
A1  - Tampé, Robert
T1  - Optical control of the antigen translocation by synthetic photo-conditional viral inhibitors
T2  - Chemical science
N2  - The immune system makes use of major histocompatibility complex class I (MHC I) molecules to present peptides to other immune cells, which can evoke an immune response. Within this process of antigen presentation, the MHC I peptide loading complex, consisting of a transporter associated with antigen processing TAP, MHC I, and chaperones, is key to the initiation of immune response by shuttling peptides from the cytosol into the ER lumen. However, it is still enigmatic how the flux of antigens is precisely coordinated in time and space, limiting our understanding of antigen presentation pathways. Here, we report on the development of a synthetic viral TAP inhibitor that can be cleaved by light. This photo-conditional inhibitor shows temporal blockade of TAP-mediated antigen translocation, which is unleashed upon illumination. The recovery of TAP activity was monitored at single-cell resolution both in human immune cell lines and primary cells. The development of a photo-conditional TAP inhibitor thus expands the repertoire of chemical intervention tools for immunological processes.
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50054
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-500541
SN  - 2041-6520
SN  - 2041-6539
N1  - This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
VL  - 10
IS  - 7
SP  - 2001
EP  - 2005
PB  - RSC
CY  - Cambridge
ER  -