TY  - JOUR
A1  - Ghiţu, Alexandra
A1  - Schwiebs, Anja
A1  - Radeke, Heinfried H.
A1  - Avram, Stefana
A1  - Zupko, Istvan
A1  - Bor, Andrea
A1  - Pavel, Ioana Zinuca
A1  - Dehelean, Cristina Adriana
A1  - Oprean, Camelia
A1  - Bojin, Florina
A1  - Farcas, Claudia
A1  - Soica, Codruta
A1  - Duicu, Oana
A1  - Danciu, Corina
T1  - A comprehensive assessment of apigenin as an antiproliferative, proapoptotic, antiangiogenic and immunomodulatory phytocompound
T2  - Nutrients
N2  - Apigenin (4′,5,7-trihydroxyflavone) (Api) is an important component of the human diet, being distributed in a wide number of fruits, vegetables and herbs with the most important sources being represented by chamomile, celery, celeriac and parsley. This study was designed for a comprehensive evaluation of Api as an antiproliferative, proapoptotic, antiangiogenic and immunomodulatory phytocompound. In the set experimental conditions, Api presents antiproliferative activity against the A375 human melanoma cell line, a G2/M arrest of the cell cycle and cytotoxic events as revealed by the lactate dehydrogenase release. Caspase 3 activity was inversely proportional to the Api tested doses, namely 30 μM and 60 μM. Phenomena of early apoptosis, late apoptosis and necrosis following incubation with Api were detected by Annexin V-PI double staining. The flavone interfered with the mitochondrial respiration by modulating both glycolytic and mitochondrial pathways for ATP production. The metabolic activity of human dendritic cells (DCs) under LPS-activation was clearly attenuated by stimulation with high concentrations of Api. Il-6 and IL-10 secretion was almost completely blocked while TNF alpha secretion was reduced by about 60%. Api elicited antiangiogenic properties in a dose-dependent manner. Both concentrations of Api influenced tumour cell growth and migration, inducing a limited tumour area inside the application ring, associated with a low number of capillaries.
KW  - apigenin
KW  - A375 human melanoma cell line
KW  - proliferation
KW  - apoptosis
KW  - mitochondrial bioenergetics and glycolysis
KW  - angiogenesis
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50063
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-500631
SN  - 2072-6643
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
VL  - 11
IS  - 4, Art. 858
SP  - 1
EP  - 19
PB  - MDPI
CY  - Basel
ER  -