TY  - JOUR
A1  - Henrich, Birgit
A1  - Hermann, Ilka
A1  - Di Giulio, Mara
A1  - Köhrer, Karl
A1  - Deenen, René
A1  - Sivalingam, Sugi
A1  - Peters, Ulrike
A1  - Beikler, Thomas
A1  - Janda, Ralf
A1  - Rüttermann, Stefan
T1  - Reexamination in vitro and in situ of an antibacterially modified experimental dental resin composite with molecular methods : a pilot study
T2  - Advances in materials science and engineering
N2  - Purpose. To introduce additional methods to detect and to quantify single pathogens in the complex biofilm formation on an antibacterial dental material.
Materials and Methods. A conventional (ST) and an antibacterial dental composite (B) were manufactured. In vitro: specimens were incubated with a mixture of early colonizers. Bacterial adhesion was analyzed by TaqMan PCR after 8/24 h. In situ: TaqMan PCR and 16S rRNA Next Generation Sequencing (NGS) were performed.
Results. In vitro: after 8 h incubation, B was covered by 58.6% of the bacterial amount that was attached to ST. After 24 h, the amount of attached bacteria to ST remained constant on ST only slightly lower on B. In situ: after 8 h the amount of adhering A. viscosus and S. mitis was prominent on ST and reduced on B. NGS revealed that S. sanguinis, S. parasanguinis, and Gemella sanguinis were the mainly attached species with S. sanguinis dominant on ST and S. parasanguinis and G. sanguinis dominant on B.
Conclusions. Initial biofilm formation was altered by B. A shift between actinomycetes and streptococci was observed in situ. TaqMan PCR and 16S rRNA NGS revealed comparable results in situ and demonstrated the usefulness of NGS to characterize complex bacterial communities.
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50653
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-506536
SN  - 1687-8442
SN  - 1687-8434
N1  - Copyright © 2016 Birgit Henrich et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 2016
IS  - Art. 6367234
SP  - 1
EP  - 12
PB  - Hindawi
CY  - New York, NY
ER  -