TY  - JOUR
A1  - Peters, Ulrike
A1  - Solominidou, Eleni
A1  - Korkmaz, Yüksel
A1  - Rüttermann, Stefan
A1  - Klocke, Astrid
A1  - Flemmig, Thomas Frank
A1  - Beikler, Thomas
T1  - Regulator of calcineurin 1 in periodontal disease
T2  - Mediators of inflammation
N2  - Nuclear factor of activated T-cells (NFAT) and NF-kB pathway associated processes are involved in the pathogenesis of various inflammatory disorders, for example, periodontal disease. The activation of these pathways is controlled by the regulator of calcineurin 1 (RCAN1). The aim of this study was to elucidate the role of RCAN1 in periodontal disease. Healthy and inflamed periodontal tissues were analyzed by immunohistochemistry and immunofluorescence using specific rabbit polyclonal anti-RCAN1 antibodies. For expression analysis human umbilical vein endothelial cells (HUVEC) were used. HUVEC were incubated for 2 h with Vascular Endothelial Growth Factor (VEGF) or with wild type and laboratory strains of Porphyromonas gingivalis (P. gingivalis). Expression analysis of rcan1 and cox2 was done by real time PCR using specific primers for rcan1.4 and cox2. The expression of rcan1 was found to be significantly suppressed in endothelial cells of chronically inflamed periodontal tissues compared to healthy controls. Rcan1 and cox2 were significantly induced by VEGF and wild type and laboratory P. gingivalis strains. Interestingly, the magnitude of the rcan1 and cox2 induction was strain dependent. The results of this study indicate that RCAN1 is suppressed in endothelial cells of chronically inflamed periodontal tissues. During an acute infection, however, rcan1 seems to be upregulated in endothelial cells, indicating a modulating role in immune homeostasis of periodontal tissues.
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50654
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-506544
SN  - 1466-1861
SN  - 0962-9351
N1  - Copyright © 2016 Ulrike Peters et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 25
IS  - Art. 5475821
SP  - 1
EP  - 7
PB  - Hindawi Publishing Corp.
CY  - Sylvania, Ohio
ER  -