TY  - JOUR
A1  - Kollmannsperger, Alina
A1  - Sharei, Armon
A1  - Raulf, Anika
A1  - Heilemann, Mike
A1  - Langer, Robert
A1  - Jensen, Klavs F.
A1  - Wieneke, Ralph
A1  - Tampé, Robert
T1  - Live-cell protein labelling with nanometre precision by cell squeezing
T2  - Nature Communications
N2  - Live-cell labelling techniques to visualize proteins with minimal disturbance are important; however, the currently available methods are limited in their labelling efficiency, specificity and cell permeability. We describe high-throughput protein labelling facilitated by minimalistic probes delivered to mammalian cells by microfluidic cell squeezing. High-affinity and target-specific tracing of proteins in various subcellular compartments is demonstrated, culminating in photoinduced labelling within live cells. Both the fine-tuned delivery of subnanomolar concentrations and the minimal size of the probe allow for live-cell super-resolution imaging with very low background and nanometre precision. This method is fast in probe delivery (∼1,000,000 cells per second), versatile across cell types and can be readily transferred to a multitude of proteins. Moreover, the technique succeeds in combination with well-established methods to gain multiplexed labelling and has demonstrated potential to precisely trace target proteins, in live mammalian cells, by super-resolution microscopy.
KW  - Cell biology
KW  - Cytological techniques
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50695
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-506950
SN  - 2041-1723
N1  - This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
VL  - 7
IS  - Art. 10372
SP  - 1
EP  - 7
PB  - Nature Publishing Group UK
CY  - [London]
ER  -