TY  - JOUR
A1  - Bresler, Ayko
A1  - Vogel, Johanna
A1  - Niederer, Daniel
A1  - Gray, Daphne
A1  - Schmitz-Rixen, Thomas
A1  - Troidl, Kerstin
T1  - Development of an exercise training protocol to investigate arteriogenesis in a murine model of peripheral artery disease
T2  - International journal of molecular sciences
N2  - Exercise is a treatment option in peripheral artery disease (PAD) patients to improve their clinical trajectory, at least in part induced by collateral growth. The ligation of the femoral artery (FAL) in mice is an established model to induce arteriogenesis. We intended to develop an animal model to stimulate collateral growth in mice through exercise. The training intensity assessment consisted of comparing two different training regimens in C57BL/6 mice, a treadmill implementing forced exercise and a free-to-access voluntary running wheel. The mice in the latter group covered a much greater distance than the former pre- and postoperatively. C57BL/6 mice and hypercholesterolemic ApoE-deficient (ApoE-/-) mice were subjected to FAL and had either access to a running wheel or were kept in motion-restricting cages (control) and hind limb perfusion was measured pre- and postoperatively at various times. Perfusion recovery in C57BL/6 mice was similar between the groups. In contrast, ApoE-/- mice showed significant differences between training and control 7 d postoperatively with a significant increase in pericollateral macrophages while the collateral diameter did not differ between training and control groups 21 d after surgery. ApoE-/- mice with running wheel training is a suitable model to simulate exercise induced collateral growth in PAD. This experimental set-up may provide a model for investigating molecular training effects.
KW  - arteriogenesis
KW  - exercise training
KW  - mouse model
KW  - femoral artery ligation
KW  - running wheel
KW  - voluntary training
KW  - peripheral artery disease
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50770
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-507702
SN  - 1422-0067
SN  - 1661-6596
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
VL  - 20
IS  - 16, Art. 3956
SP  - 1
EP  - 12
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -