TY  - JOUR
A1  - Lingaraju, Mahesh
A1  - Johnsen, Dennis
A1  - Schlundt, Andreas
A1  - Langer, Lukas M.
A1  - Basquin, Jérôme
A1  - Sattler, Michael
A1  - Jensen, Torben Heick
A1  - Falk, Sebastian
A1  - Conti, Elena
T1  - The MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs
T2  - Nature Communications
N2  - The nuclear exosome and its essential co-factor, the RNA helicase MTR4, play crucial roles in several RNA degradation pathways. Besides unwinding RNA substrates for exosome-mediated degradation, MTR4 associates with RNA-binding proteins that function as adaptors in different RNA processing and decay pathways. Here, we identify and characterize the interactions of human MTR4 with a ribosome processing adaptor, NVL, and with ZCCHC8, an adaptor involved in the decay of small nuclear RNAs. We show that the unstructured regions of NVL and ZCCHC8 contain short linear motifs that bind the MTR4 arch domain in a mutually exclusive manner. These short sequences diverged from the arch-interacting motif (AIM) of yeast rRNA processing factors. Our results suggest that nuclear exosome adaptors have evolved canonical and non-canonical AIM sequences to target human MTR4 and demonstrate the versatility and specificity with which the MTR4 arch domain can recruit a repertoire of different RNA-binding proteins.
KW  - NMR spectroscopy
KW  - RNA
KW  - RNA decay
KW  - X-ray crystallography
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50870
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-508706
SN  - 2041-1723
N1  - Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
VL  - 10
IS  - 1, Art. 3393
SP  - 1
EP  - 11
PB  - Nature Publishing Group UK
CY  - [London]
ER  -