TY  - JOUR
A1  - Schanzenbächer, Christoph Tobias
A1  - Langer, Julian David
A1  - Schuman, Erin
T1  - Time- and polarity-dependent proteomic changes associated with homeostatic scaling at central synapses
T2  - eLife
N2  - In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells with either TTX or bicucculline for 2 hr to induce the process of up- or down-scaling, respectively. During the activity manipulation we metabolically labeled newly synthesized proteins using BONCAT. We identified 168 newly synthesized proteins that exhibited significant changes in expression. To obtain a temporal trajectory of the response, we compared the proteins synthesized within 2 hr or 24 hr of the activity manipulation. Surprisingly, there was little overlap in the significantly regulated newly synthesized proteins identified in the early- and integrated late response datasets. There was, however, overlap in the functional categories that are modulated early and late. These data indicate that within protein function groups, different proteomic choices can be made to effect early and late homeostatic responses that detect the duration and polarity of the activity manipulation.
KW  - Research article
KW  - Cell biology
KW  - neuroscience
KW  - proteomics
KW  - homeostasis
KW  - synaptic plasticity
KW  - synaptic scaling
KW  - rat
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51008
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-510083
SN  - 2050-084X
N1  - Copyright Schanzenbächer et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
VL  - 7
IS  - e33322
SP  - 1
EP  - 20
PB  - eLife Sciences Publications
CY  - Cambridge
ER  -