TY  - JOUR
A1  - Marko, Dominik
A1  - El-Shershaby, Asmaa Samir Attia
A1  - Carriero, Filomena
A1  - Summerer, Stephan
A1  - Petrozza, Angelo
A1  - Iannacone, Rina
A1  - Schleiff, Enrico
A1  - Fragkostefanakis, Sotirios
T1  - Identification and characterization of a thermotolerant tilling allele of heat shock binding protein 1 in tomato
T2  - Genes
N2  - The identification of heat stress (HS)-resilient germplasm is important to ensure food security under less favorable environmental conditions. For that, germplasm with an altered activity of factors regulating the HS response is an important genetic tool for crop improvement. Heat shock binding protein (HSBP) is one of the main negative regulators of HS response, acting as a repressor of the activity of HS transcription factors. We identified a TILLING allele of Solanum lycopersicum (tomato) HSBP1. We examined the effects of the mutation on the functionality of the protein in tomato protoplasts, and compared the thermotolerance capacity of lines carrying the wild-type and mutant alleles of HSBP1. The methionine-to-isoleucine mutation in the central heptad repeats of HSBP1 leads to a partial loss of protein function, thereby reducing the inhibitory effect on Hsf activity. Mutant seedlings show enhanced basal thermotolerance, while mature plants exhibit increased resilience in repeated HS treatments, as shown by several physiological parameters. Importantly, plants that are homozygous for the wild-type or mutant HSBP1 alleles showed no significant differences under non-stressed conditions. Altogether, these results indicate that the identified mutant HSBP1 allele can be used as a genetic tool in breeding, aiming to improve the thermotolerance of tomato varieties.
KW  - Solanum lycopersicum
KW  - heat stress
KW  - thermotolerance
KW  - heat shock protein
KW  - heat stress transcription factor
KW  - phenotyping
KW  - HSBP
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51268
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-512683
SN  - 2073-4425
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 10
IS  - 7, Art. 516
SP  - 1
EP  - 17
PB  - MDPI
CY  - Basel
ER  -