TY  - JOUR
A1  - Wunnicke, Dorith
A1  - Hänelt, Inga
T1  - The synergetic effects of combining structural biology and epr spectroscopy on membrane proteins
T2  - Crystals
N2  - Protein structures as provided by structural biology such as X-ray crystallography, cryo-electron microscopy and NMR spectroscopy are key elements to understand the function of a protein on the molecular level. Nonetheless, they might be error-prone due to crystallization artifacts or, in particular in case of membrane-imbedded proteins, a mostly artificial environment. In this review, we will introduce different EPR spectroscopy methods as powerful tools to complement and validate structural data gaining insights in the dynamics of proteins and protein complexes such that functional cycles can be derived. We will highlight the use of EPR spectroscopy on membrane-embedded proteins and protein complexes ranging from receptors to secondary active transporters as structural information is still limited in this field and the lipid environment is a particular challenge.
KW  - EPR spectroscopy
KW  - PELDOR
KW  - structural biology
KW  - membrane proteins
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51465
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-514656
SN  - 2073-4352
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 7
IS  - 4, Art. 117
SP  - 1
EP  - 29
PB  - MDPI
CY  - Basel
ER  -