TY  - JOUR
A1  - Tascher, Georg
A1  - Burban, Audrey
A1  - Camus, Sandrine
A1  - Plumel, Marine
A1  - Chanon, Stéphanie
A1  - Le Guével, Rémy
A1  - Shevchenko, Valery
A1  - Van Dorsselaer, Alain
A1  - Lefai, Etienne
A1  - Guguen-Guillouzo, Christiane
A1  - Bertile, Fabrice
T1  - In-depth proteome analysis highlights heparg cells as a versatile cell system surrogate for primary human hepatocytes
T2  - Cells
N2  - Of the hepatic cell lines developed for in vitro studies of hepatic functions as alternatives to primary human hepatocytes, many have lost major liver-like functions, but not HepaRG cells. The increasing use of the latter worldwide raises the need for establishing the reference functional status of early biobanked HepaRG cells. Using deep proteome and secretome analyses, the levels of master regulators of the hepatic phenotype and of the structural elements ensuring biliary polarity were found to be close to those in primary hepatocytes. HepaRG cells proved to be highly differentiated, with functional mitochondria, hepatokine secretion abilities, and an adequate response to insulin. Among differences between primary human hepatocytes and HepaRG cells, the factors that possibly support HepaRG transdifferentiation properties are discussed. The HepaRG cell system thus appears as a robust surrogate for primary hepatocytes, which is versatile enough to study not only xenobiotic detoxification, but also the control of hepatic energy metabolism, secretory function and disease-related mechanisms.
KW  - Hepatocytes
KW  - liver cell lines
KW  - HepaRG cells
KW  - proteome
KW  - secretome
KW  - hepatic phenotype
KW  - detoxification
KW  - liver metabolism
KW  - liver diseases
KW  - transdifferentiation
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51468
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-514682
SN  - 2073-4409
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 8
IS  - 2, Art. 192
SP  - 1
EP  - 25
PB  - MDPI
CY  - Basel
ER  -