TY  - JOUR
A1  - Minh, Truong Ngoc
A1  - Xuan, Tran Dang
A1  - Ahmad, Ateeque
A1  - Elzaawely, Abdelnaser Abdelghany
A1  - Teschke, Rolf
A1  - Van, Truong Mai
T1  - Momilactones a and b: Optimization of yields from isolation and purification
T2  - Separations
N2  - Momilactones A and B (MA and MB, respectively) are phytoalexins and plant growth inhibitors available in rice husks. However, to date, the isolation and purification of the two compounds have been complicated, laborious, and less effective. The present study was conducted to establish a protocol to simplify and optimize quantities of MA and MB by combinations of ethyl acetate (EtOAc), distilled water, methanol (MeOH), temperature, and pressure for extractions. At a temperature of 100 °C, combined with EtOAc and MeOH 100%, MA and MB were enriched in greater quantities than non-treated rice husks, of which MB was much augmented than MA. The EtOAc extract obtained from samples dried at 100 °C for 1 h, then placed in MeOH 100% for 1 week, provided maximum yields of MA [58.76 µg/g dry weight (DW)] and MB (104.43 µg/g DW). The use of pressure effectively enhanced yields of MA (17.90–26.26 µg/g DW) and MB (40.78–71.0 µg/g DW). The actual purified amounts of MA and MB increased by 5 and 15 folds, respectively. The use of either sole distilled water or MeOH ≤ 50% at any temperature did not successfully isolate both MA and MB. The yield optimization aids to easier and more productive purification of the two compounds, and thus extends researches on biological activities of MA and MB, including pharmaceutical and medicinal properties.
KW  - momilactone A
KW  - momilactone B
KW  - column chromatography
KW  - yield optimization
KW  - temperature
KW  - pressure
KW  - extracting solvents
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51486
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-514867
SN  - 2297-8739
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 5
IS  - 2, Art. 28
SP  - 1
EP  - 10
PB  - MDPI
CY  - Basel
ER  -