TY  - JOUR
A1  - Dutta, Priyanka
A1  - Odedra, Devang
A1  - Pohl, Christian
T1  - Planar asymmetries in the C. elegans embryo emerge by differential retention of apars at cell-cell contacts
T2  - Frontiers in cell and developmental biology
N2  - Formation of the anteroposterior and dorsoventral body axis in Caenorhabditis elegans depends on cortical flows and advection of polarity determinants. The role of this patterning mechanism in tissue polarization after formation of cell-cell contacts is not fully understood. Here, we demonstrate that planar asymmetries are established during left-right symmetry breaking: Centripetal cortical flows asymmetrically and differentially advect anterior polarity determinants (aPARs) from contacts to the medial cortex, resulting in their unmixing from apical myosin. Contact localization and advection of PAR-6 requires balanced CDC-42 activation, while asymmetric retention and advection of PAR-3 can occur independently of PAR-6. Concurrent asymmetric retention of PAR-3, E-cadherin/HMR-1 and opposing retention of antagonistic CDC-42 and Wnt pathway components leads to planar asymmetries. The most obvious mark of planar asymmetry, retention of PAR-3 at a single cell-cell contact, is required for proper cytokinetic cell intercalation. Hence, our data uncover how planar polarity is established in a system without the canonical planar cell polarity pathway through planar asymmetric retention of aPARs.
KW  - planar polarity
KW  - asymmetry
KW  - cortical flow
KW  - PAR complex
KW  - CDC-42
KW  - morphogenesis
KW  - Wnt
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51524
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-515247
SN  - 2296-634X
N1  - Copyright © 2019 Dutta, Odedra and Pohl. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
VL  - 7
IS  - Art. 209
SP  - 1
EP  - 20
PB  - Frontiers Media
CY  - Lausanne
ER  -