TY  - JOUR
A1  - Lehrnbecher, Thomas
A1  - Rath, Peter
A1  - Attarbaschi, Andishe
A1  - Cario, Gunnar
A1  - Döring, Michaela
A1  - Moser, Olga
A1  - Mücke, Urs
A1  - Poyer, Fiona
A1  - Rieken, Sarah
A1  - Temme, Christian
A1  - Voigt, Sebastian
A1  - Groll, Andreas Hermann
A1  - Lauten, Melchior Johannes
T1  - Galactomannan and pcr in the central nervous system to detect invasive mold disease - a retrospective analysis in immunocompromised children
T2  - Scientific reports
N2  - Invasive mold disease (IMD) of the central nervous system (CNS) is a severe infectious complication in immunocompromised patients, but early microbiological diagnosis is difficult. As data on the value of biomarkers in the CNS are scarce, in particular in children, we retrospectively analyzed the performance of galactomannan (GM) and PCR assays in CNS samples of 15 children with proven and probable CNS IMD and of 32 immunocompromised children without fungal infection. Galactomannan in the cerebrospinal fluid (CSF) was assessed in nine of the 15 pediatric patients and was positive in five of them. Polymerase chain reaction (PCR) was performed in eight of the 15 patients and detected nucleic acids from molds in six patients. Galactomannan and PCR in CNS samples were the only positive microbiologic parameter in the CNS in three and two patients, respectively. In four patients, PCR specified the pathogen detected in microscopy. Galactomannan and PCR results remained negative in the CSF of all immunocompromised children without evidence for CNS IMD. Our data suggest that GM and PCR in CNS specimens are valuable additional tools in diagnosing CNS IMD and should be included in the work up of all pediatric patients with suspected mold disease of the CNS.
KW  - Diagnostic markers
KW  - Paediatric research
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51993
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-519937
SN  - 2045-2322
N1  - Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
VL  - 9
IS  - 1, Art. 12950
SP  - 1
EP  - 5
PB  - Macmillan Publishers Limited, part of Springer Nature
CY  - [London]
ER  -