TY  - JOUR
A1  - Buaya, Anthony Tumenez
A1  - Kraberg, Alexandra
A1  - Thines, Marco
T1  - Dual culture of the oomycete Lagenisma coscinodisci Drebes and Coscinodiscus diatoms as a model for plankton/parasite interactions
T2  - Helgoland marine research
N2  - Diatoms are thought to provide about 40% of total global photosynthesis and diatoms of the genus Coscinodiscus are an important, sometimes dominant, cosmopolitan component of the marine diatom community. The oomycete parasitoid Lagenisma coscinodisci is widespread in the northern hemisphere on its hosts in the genus Coscinodiscus. Because of its potential ecological importance, it would be a suitable pathogen model to investigate plankton/parasite interactions, but the species cannot be cultivated on media without its host, so far. Thus, it was the aim of this study to explore the potential of dual culture of host and pathogen in the laboratory and to optimise cultivation to ensure a long-term cultivation of the pathogen. Here, we report successful cultivation of a single spore strain of L. coscinodisci (Isla), on several Coscinodiscus species and strains, as well as the establishment of a cultivation routine with Coscinodiscus granii (CGS1 and CG36), which enabled us to maintain the single spore strain for more than 3 years in 6 cm Petri dishes and 10 ml tissue culture flasks. This opens up the opportunity to study the processes and mechanism in plankton/parasitoid interactions under controlled conditions.
KW  - Centric diatom
KW  - Coscinodiscus concinnus
KW  - C. grani
KW  - C. radiatus
KW  - C. wailesii
KW  - Cultivation
KW  - Laboratory
KW  - Oomycota
KW  - Pathosystem
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55343
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-553436
SN  - 1438-3888
SN  - 1438-387X
VL  - 73
IS  - 1
SP  - 1
EP  - 6
PB  - BioMed Central
ER  -