TY  - JOUR
A1  - Kohmer, Niko
A1  - Toptan, Tuna
A1  - Pallas, Christiane
A1  - Karaca, Onur
A1  - Pfeiffer, Annika Eby
A1  - Westhaus, Sandra
A1  - Widera, Marek
A1  - Berger, Annemarie
A1  - Hoehl, Sebastian
A1  - Kammel, Martin
A1  - Ciesek, Sandra
A1  - Rabenau, Holger
T1  - The comparative clinical performance of four SARS-CoV-2 rapid antigen tests and their correlation to infectivity in vitro
T2  - Journal of clinical medicine
N2  - Due to globally rising numbers of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, resources for real-time reverse-transcription polymerase chain reaction (rRT-PCR)-based testing have been exhausted. In order to meet the demands of testing and reduce transmission, SARS-CoV-2 antigen-detecting rapid diagnostic tests (Ag-RDTs) are being considered. These tests are fast, inexpensive, and simple to use, but whether they detect potentially infectious cases has not been well studied. We evaluated three lateral flow assays (RIDA®QUICK SARS-CoV-2 Antigen (R-Biopharm), SARS-CoV-2 Rapid Antigen Test (Roche)), and NADAL® COVID-19 Ag Test (Nal von Minden GmbH, Regensburg, Germany) and one microfluidic immunofluorescence assay (SARS-CoV-2 Ag Test (LumiraDx GmbH, Cologne, Germany)) using 100 clinical samples. Diagnostic rRT-PCR and cell culture testing as a marker for infectivity were performed in parallel. The overall Ag-RDT sensitivity for rRT-PCR-positive samples ranged from 24.3% to 50%. However, for samples with a viral load of more than 6 log10 RNA copies/mL (22/100), typically seen in infectious individuals, Ag-RDT positivity was between 81.8% and 100%. Only 51.6% (33/64) of the rRT-PCR-positive samples were infectious in cell culture. In contrast, three Ag-RDTs demonstrated a more significant correlation with cell culture infectivity (61.8–82.4%). Our findings suggest that large-scale SARS-CoV-2 Ag-RDT-based testing can be considered for detecting potentially infective individuals and reducing the virus spread.
KW  - SARS-CoV-2
KW  - POCT
KW  - Ag-RDT
KW  - PCR
KW  - cell culture
KW  - infectivity
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/59365
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-593653
SN  - 2077-0383
N1  - Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
VL  - 10
IS  - 2, art. 328
SP  - 1
EP  - 11
PB  - MDPI
CY  - Basel
ER  -