TY  - JOUR
A1  - Wittig, Ilka
A1  - Malacarne, Pedro Felipe
T1  - Complexome profiling: assembly and remodeling of protein complexes
T2  - International journal of molecular sciences
N2  - Many proteins have been found to operate in a complex with various biomolecules such as proteins, nucleic acids, carbohydrates, or lipids. Protein complexes can be transient, stable or dynamic and their association is controlled under variable cellular conditions. Complexome profiling is a recently developed mass spectrometry-based method that combines mild separation techniques, native gel electrophoresis, and density gradient centrifugation with quantitative mass spectrometry to generate inventories of protein assemblies within a cell or subcellular fraction. This review summarizes applications of complexome profiling with respect to assembly ranging from single subunits to large macromolecular complexes, as well as their stability, and remodeling in health and disease.
KW  - complexome profiling
KW  - mass spectrometry
KW  - protein complexes
KW  - protein–protein interaction
KW  - assembly
KW  - remodeling
KW  - data repositories
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/61895
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-618958
SN  - 1422-0067
VL  - 22
IS  - 15, art. 7809
SP  - 1
EP  - 12
PB  - MDPI
CY  - Basel
ER  -