TY  - JOUR
A1  - Spahn, Christoph Klaus
A1  - Hurter, Florian
A1  - Glaesmann, Mathilda
A1  - Karathanasis, Christos
A1  - Lampe, Marko
A1  - Heilemann, Mike
T1  - Protein-specific, multicolor and 3D STED imaging in cells with DNA-labeled antibodies
T2  - Angewandte Chemie
N2  - Photobleaching is a major challenge in fluorescence microscopy, in particular if high excitation light intensities are used. Signal-to-noise and spatial resolution may be compromised, which limits the amount of information that can be extracted from an image. Photobleaching can be bypassed by using exchangeable labels, which transiently bind to and dissociate from a target, thereby replenishing the destroyed labels with intact ones from a reservoir. Here, we demonstrate confocal and STED microscopy with short, fluorophore-labeled oligonucleotides that transiently bind to complementary oligonucleotides attached to protein-specific antibodies. The constant exchange of fluorophore labels in DNA-based STED imaging bypasses photobleaching that occurs with covalent labels. We show that this concept is suitable for targeted, two-color STED imaging of whole cells.
KW  - DNA-PAINT
KW  - fluorescence
KW  - fluorescent probes
KW  - multicolor imaging
KW  - STED microscopy
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63817
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-638176
SN  - 1521-3773
N1  - M.H., M.G., C.K., and C.S. acknowledge funding by the German Science Foundation (SFB 902, SFB 807, HE6166/17-1, HE6166/11-1).
VL  - 58
IS  - 52
SP  - 18835
EP  - 18838
PB  - Wiley-VCH
CY  - Weinheim
ER  -