TY - JOUR A1 - Bode, Edna A1 - Heinrich, Antje Katharina A1 - Hirschmann, Merle A1 - Abebew, Desalegne A1 - Shi, Yan-Ni A1 - Vo, Tien Duy A1 - Wesche, Frank A1 - Shi, Yi-Ming A1 - Grün, Peter A1 - Simonyi, Svenja A1 - Keller, Nadine A1 - Engel, Yvonne A1 - Wenski, Sebastian A1 - Bennet, Reuel A1 - Beyer, Sophie A1 - Bischoff, Iris A1 - Buaya, Anthony Tumenez A1 - Brandt, Sophie A1 - Cakmak, Ibrahim A1 - Çimen, Harun A1 - Eckstein, Simone A1 - Frank, Denia A1 - Fürst, Robert A1 - Gand, Martin A1 - Geisslinger, Gerd A1 - Hazir, Selcuk A1 - Henke, Marina A1 - Heermann, Ralf A1 - Lecaudey, Virginie A1 - Schäfer, Wilhelm A1 - Schiffmann, Susanne A1 - Schüffler, Anja A1 - Schwenk, Rebecca A1 - Skaljac, Marisa A1 - Thines, Eckhard A1 - Thines, Marco A1 - Ulshöfer, Thomas A1 - Vilcinskas, Andreas A1 - Wichelhaus, Thomas Alexander A1 - Bode, Helge Björn T1 - Promoter activation in Δhfq mutants as an efficient tool for specialized metabolite production enabling direct bioactivity testing T2 - Angewandte Chemie N2 - Natural products (NPs) from microorganisms have been important sources for discovering new therapeutic and chemical entities. While their corresponding biosynthetic gene clusters (BGCs) can be easily identified by gene-sequence-similarity-based bioinformatics strategies, the actual access to these NPs for structure elucidation and bioactivity testing remains difficult. Deletion of the gene encoding the RNA chaperone, Hfq, results in strains losing the production of most NPs. By exchanging the native promoter of a desired BGC against an inducible promoter in Δhfq mutants, almost exclusive production of the corresponding NP from the targeted BGC in Photorhabdus, Xenorhabdus and Pseudomonas was observed including the production of several new NPs derived from previously uncharacterized non-ribosomal peptide synthetases (NRPS). This easyPACId approach (easy Promoter Activated Compound Identification) facilitates NP identification due to low interference from other NPs. Moreover, it allows direct bioactivity testing of supernatants containing secreted NPs, without laborious purification. KW - bioactivity testing KW - easyPACId KW - natural products KW - proteobacteria KW - simplified production Y1 - 2019 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63820 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-638202 SN - 1521-3773 N1 - Research Funding: LOEWE-Centre Translational Biodiversity Genomics. Grant Number: LOEWE TBG; Scientific and Technological Research Council of Turkey (TUBITAK). Grant Number: TOVAG-117O172; LOEWE-Centre Translational Medicine and Pharmacology. Grant Number: LOEWE TMP; LOEWE-Centre Insect Biotechnology and Bioressources. Grant Number: LOEWE Insect biotechnology; LOEWE research cluster MegaSyn. Grant Number: LOEWE MegaSyn; DFG. Grant Number: INST 161/810-1. VL - 58 IS - 52 SP - 18957 EP - 18963 PB - Wiley-VCH CY - Weinheim ER -