TY  - JOUR
A1  - Nürenberg-Goloub, Elina
A1  - Kratzat, Hanna
A1  - Heinemann, Holger
A1  - Heuer, André
A1  - Kötter, Peter
A1  - Berninghausen, Otto
A1  - Becker, Thomas
A1  - Tampé, Robert
A1  - Beckmann, Roland
T1  - Molecular analysis of the ribosome recycling factor ABCE1 bound to the 30S post-splitting complex
T2  - The EMBO journal
N2  - Ribosome recycling by the twin-ATPase ABCE1 is a key regulatory process in mRNA translation and surveillance and in ribosome-associated protein quality control in Eukarya and Archaea. Here, we captured the archaeal 30S ribosome post-splitting complex at 2.8 Å resolution by cryo-electron microscopy. The structure reveals the dynamic behavior of structural motifs unique to ABCE1, which ultimately leads to ribosome splitting. More specifically, we provide molecular details on how conformational rearrangements of the iron–sulfur cluster domain and hinge regions of ABCE1 are linked to closure of its nucleotide-binding sites. The combination of mutational and functional analyses uncovers an intricate allosteric network between the ribosome, regulatory domains of ABCE1, and its two structurally and functionally asymmetric ATP-binding sites. Based on these data, we propose a refined model of how signals from the ribosome are integrated into the ATPase cycle of ABCE1 to orchestrate ribosome recycling.
KW  - ABC proteins, ribosome recycling
KW  - molecular machines
KW  - mRNA surveillance
KW  - ribosome-associated quality control
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63826
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-638262
SN  - 1460-2075
N1  - E.N.G. was supported by the Christiane Nüsslein-Volhard Foundation, L'Oréal, and the United Nations Educational, Scientific and Cultural Organization (UNESCO). H.K. is supported by a DFG fellowship through the Graduate School of Quantitative Bioscience Munich (QBM). The German Research Foundation (DFG) SFB 902 “Molecular mechanisms of RNA-based regulation” (to R.T.), TRR174 “Spatiotemporal dynamics of bacterial cells” (to R.B.) and FOR 1805 (to R.B.) funded this work.
VL  - 39
IS  - 9, art. e103788
SP  - 1
EP  - 13
PB  - EMBO Press; Wiley
CY  - Heidelberg; Hoboken, NJ [u.a.]
ER  -