TY  - JOUR
A1  - Müller, Diana
A1  - Trucks, Sven
A1  - Schwalbe, Harald
A1  - Hengesbach, Martin
T1  - Genetic code expansion facilitates position-selective modification of nucleic acids and proteins
T2  - ChemPlusChem
N2  - Transcription and translation obey to the genetic code of four nucleobases and 21 amino acids evolved over billions of years. Both these processes have been engineered to facilitate the use of non-natural building blocks in both nucleic acids and proteins, enabling researchers with a decent toolbox for structural and functional analyses. Here, we review the most common approaches for how labeling of both nucleic acids as well as proteins in a site-selective fashion with either modifiable building blocks or spectroscopic probes can be facilitated by genetic code expansion. We emphasize methodological approaches and how these can be adapted for specific modifications, both during as well as after biomolecule synthesis. These modifications can facilitate, for example, a number of different spectroscopic analysis techniques and can under specific circumstances even be used in combination.
KW  - fluorescence
KW  - genetic code expansion
KW  - non-canonical amino acid
KW  - non-natural nucleotide
KW  - site-specific labeling
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63832
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-638323
SN  - 2192-6506
N1  - Experiments were supported by DFG through collaborative research center 902: Molecular principles of RNA-based regulation and individual grant SCHW701/22-1. BMRZ is supported by the state of Hesse.
VL  - 85
IS  - 6
SP  - 1233
EP  - 1243
PB  - Wiley-VCH
CY  - Weinheim
ER  -