TY  - THES
A1  - Kolbe, Felix Martin
T1  - Structural insights into the catalytic binuclear center of cytochrome c oxidase from Paracoccus denitrificans
N2  - Cytochrome c oxidases are among the most important and fundamental enzymes of life. Integrated into membranes they use four electrons from cytochrome c molecules to reduce molecular oxygen (dioxygen) to water. Their catalytic cycle has been considered to start with the oxidized form. Subsequent electron transfers lead to the E-state, the R-state (which binds oxygen), the P-state (with an already split dioxygen bond), the F-state and the O-state again. Here, we determined structures of up to 1.9 Å resolution of these intermediates by single particle cryo-EM. Our results suggest that in the O-state the active site contains a peroxide dianion and in the P-state possibly an intact dioxygen molecule, the F-state may contain a superoxide anion.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/66955
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-669553
CY  - Frankfurt am Main
ER  -