Vaccinia virus replication is not affected by APOBEC3 family members

  • Background: The APOBEC3G protein represents a novel innate defense mechanism against retroviral infection. It facilitates the deamination of the cytosine residues in the single stranded cDNA intermediate during early steps of retroviral infection. Most poxvirus genomes are relatively A/T-rich, which may indicate APOBEC3G-induced mutational pressure. In addition, poxviruses replicate exclusively in the cytoplasm where APOBEC3G is located. It was therefore tempting to analyze whether vaccinia virus replication is affected by APOBEC3G. Results: The replication of vaccinia virus, a prototype poxvirus, was not, however, inhibited in APOBEC3G-expressing cells, nor did other members of the APOBEC3 family alter vaccinia virus replication. HIV counteracts APOBEC3G by inducing its degradation. However, Western blot analysis showed that the levels of APOBEC3G protein were not affected by vaccinia virus infection. Conclusion: The data indicate that APOBEC3G is not a restriction factor for vaccinia virus replication nor is vaccinia virus able to degrade APOBEC3G.
Author:Melanie Kremer, Yasemin Suezer, Yolanda Martinez-Fernandez, Carsten Münk, Gerd Sutter, Barbara SchnierleORCiDGND
Pubmed Id:
Parent Title (English):Virology Journal
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Date of Publication (online):2007/05/30
Year of first Publication:2006
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2007/05/30
Page Number:8
First Page:1
Last Page:8
© 2006 Kremer et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source:Virology Journal 2006, 3:86. - doi:10.1186/1743-422X-3-86 ;
Institutes:keine Angabe Fachbereich / keine Angabe Institut
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 2.0