• search hit 4 of 4
Back to Result List

The G2A receptor controls polarization of macrophage by determining their localization within the inflamed tissue

  • Macrophages are highly versatile cells, which acquire, depending on their microenvironment, pro- (M1-like), or antiinflammatory (M2-like) phenotypes. Here, we studied the role of the G-protein coupled receptor G2A (GPR132), in chemotactic migration and polarization of macrophages, using the zymosan-model of acute inflammation. G2A-deficient mice showed a reduced zymosan-induced thermal hyperalgesia, which was reversed after macrophage depletion. Fittingly, the number of M1-like macrophages was reduced in the inflamed tissue in G2A-deficient mice. However, G2A activation was not sufficient to promote M1-polarization in bone marrow-derived macrophages. While the number of monocyte-derived macrophages in the inflamed paw was not altered, G2A-deficient mice had less macrophages in the direct vicinity of the origin of inflammation, an area marked by the presence of zymosan, neutrophil accumulation and proinflammatory cytokines. Fittingly neutrophil efferocytosis was decreased in G2A-deficient mice and several lipids, which are released by neutrophils and promote G2A-mediated chemotaxis, were increased in the inflamed tissue. Taken together, G2A is necessary to position macrophages in the proinflammatory microenvironment surrounding the center of inflammation. In absence of G2A the macrophages are localized in an antiinflammatory microenvironment and macrophage polarization is shifted toward M2-like macrophages.

Download full text files

Export metadata

Metadaten
Author:Katharina Kern, Stephan M. G. Schäfer, Jennifer Cohnen, Sandra PierreORCiD, Tabea Osthues, Neda Tarighi, Stefan Hohmann, Nerea Ferreirós BouzasORCiDGND, Bernhard BrüneORCiD, Andreas WeigertORCiDGND, Gerd GeisslingerORCiDGND, Marco SisignanoORCiDGND, Klaus ScholichORCiD
URN:urn:nbn:de:hebis:30:3-474554
DOI:https://doi.org/10.3389/fimmu.2018.02261
ISSN:1664-3224
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/30327654
Parent Title (English):Frontiers in immunology
Publisher:Frontiers Media
Place of publication:Lausanne
Contributor(s):Alexandre Corthay
Document Type:Article
Language:English
Year of Completion:2018
Date of first Publication:2018/10/01
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/11/06
Tag:G2A; GPCR; acute inflammation; macrophage; migration; pain; polarization
Volume:9
Issue:Art. 2261
Page Number:12
First Page:1
Last Page:12
Note:
Copyright © 2018 Kern, Schäfer, Cohnen, Pierre, Osthues, Tarighi, Hohmann, Ferreiros, Brüne, Weigert, Geisslinger, Sisignano and Scholich. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
HeBIS-PPN:44066408X
Institutes:Medizin / Medizin
Wissenschaftliche Zentren und koordinierte Programme / Sonderforschungsbereiche / Forschungskollegs
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Open-Access-Publikationsfonds:Medizin
Licence (German):License LogoCreative Commons - Namensnennung 4.0