Enhanced ROS-generation in lymphocytes from Alzheimer’s patients

  • Introduction: Reactive oxygen species (ROS) have been implicated in neurodegeneration and seem to be involved in the physiology and pathophysiology of several diseases, including normal aging and Alzheimer’s disease (AD). Enhanced ROS production in aging or AD is not restricted to the brain, but can also been seen in several peripheral tissues. The objective of the present study was to evaluate whether the mechanisms involved in the generation of oxidative stress in normal senescence and Alzheimer’s disease are identical or not. Methods: We analysed intracellular basal levels of ROS in lymphocytes from AD patients and healthy young and aged not-demented subjects as well as ROS levels following stimulation with d-ribose and staurosporine in all three groups. ROS levels were measured by flow cytometry using the intracellular fluorescence dye dihydrorhodamine123 (DHR123). Results: Our study shows that AD lymphocytes have increased basal levels of ROS, low susceptibility to ROS stimulation by 2-deoxy-D-ribose (dRib) and an increased response to staurosporine when compared with age-matched controls. Discussion: The data suggest that the defect(s) responsible for enhanced ROS production in AD may involve different or additional biological pathways than those involved in enhanced ROS generation during aging.

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Metadaten
Author:Silke Leutner, Katharina Schindowski, Lutz Frölich, Konrad Maurer, Tilmann Kratzsch, Anne Eckert, Walter E. Müller
URN:urn:nbn:de:hebis:30-34508
DOI:https://doi.org/10.1055/s-2005-916186
Parent Title (German):Pharmacopsychiatry
Document Type:Article
Language:English
Year of Completion:2005
Year of first Publication:2005
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2006/11/29
Volume:38
Page Number:4
First Page:312
Last Page:315
Source:In: Pharmacopsychiatry (2005), 38:312-315 ; 10.1055/s-2005-916186 ; http://www.thieme-connect.com/ejournals/abstract/pharmaco/doi/10.1055/s-2005-916186
HeBIS-PPN:188868461
Institutes:Biochemie, Chemie und Pharmazie / Pharmazie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoDeutsches Urheberrecht