Allelism of PSO4 and PRP19 links pre-mRNA processing with recombination and error-prone DNA repair in Saccharomyces cerevisia
- The radiation-sensitive mutant pso4-1 of Saccharomyces cerevisiae shows a pleiotropic phenotype, including sensitivity to DNA cross-linking agents, nearly blocked sporulation and reduced mutability. We have cloned the putative yeast DNA repair gene PSO4 from a genomic library by complementation of the blocked UV-induced mutagenesis and of sporulation in diploids homozygous for pso4-1. Sequence analysis revealed that gene PSO4 consists of 1512 bp located upstream of UBI4 on chromosome XII and encodes a putative protein of 56.7 kDa. PSO4 is allelic to PRP19, a gene encoding a spliceosome-associated protein, but shares no significant homology with other yeast genes. Gene disruption with a destroyed reading frame of our PSO4 clone resulted in death of haploid cells, confirming the finding that PSO4/PRP19 is an essential gene. Thus, PSO4 is the third essential DNA repair gene found in the yeast S.cerevisiae.
| Author: | Martin Grey, Andreas Düsterhöft, Joao A. P. Henriques, Martin Brendel |
|---|---|
| URN: | urn:nbn:de:hebis:30-32711 |
| DOI: | https://doi.org/10.1093/nar/24.20.4009 |
| ISSN: | 1362-4962 |
| ISSN: | 0305-1048 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/8918805 |
| Parent Title (English): | Nucleic acids research |
| Publisher: | Oxford Univ. Press |
| Place of publication: | Oxford |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 1996 |
| Date of first Publication: | 1996/10/01 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2006/11/07 |
| Volume: | 24 |
| Issue: | 20 |
| Page Number: | 6 |
| First Page: | 4009 |
| Last Page: | 4014 |
| Note: | Copyright © 1996 by Oxford University Press, http://nar.oxfordjournals.org/misc/terms.dtl |
| Source: | Nucleic Acids Research, Vol 24 (1996), Issue 20, S. 4009-4014, http://nar.oxfordjournals.org/cgi/content/abstract/24/20/4009 |
| HeBIS-PPN: | 19232098X |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Sammlung Biologie / Sondersammelgebiets-Volltexte |
| Licence (German): |  Deutsches Urheberrecht |
