Genetic engineering of baker’s and wine yeasts using formaldehyde hyperresistance-mediating plasmids

  • Yeast multi-copy vectors carrying the for maldehyde-resistance marker gene SFA have proved to be a valuable tool for research on industrially used strains of Saccharomyces cerevisiae. The genetics of these strains is often poorly understood, and for various reasons it is not possible to simply subject these strains to protocols of genetic engineering that have been established for laboratory strains of S. cerevisiae. We tested our vectors and protocols using 10 randomly picked baker’s and wine yeasts all of which could be transformed by a simple protocol with vectors conferring hyperresistance to formaldehyde. The application of formaldehyde as a selecting agent also offers the advantage of its biodegradation to CO2 during fermentation, i.e., the selecting agent will be consumed and therefore its removal during down-stream processing is not necessary. Thus, this vector provides an expression system which is simple to apply and inexpensive to use. Key words: · Yeast · Transformation · Hyperresistance to formaldehyde

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Metadaten
Author:Martin Schmidt, Alp Cömer, Martin Grey, Martin Brendel
URN:urn:nbn:de:hebis:30-71021
URL:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200004
Parent Title (German):Brazilian Journal of Medical and Biological Research
Document Type:Article
Language:English
Date of Publication (online):2009/10/06
Year of first Publication:1997
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2009/10/06
Tag:Hyperresistance to formaldehyde; Transformation; Yeast
Volume:30
Issue:12
First Page:1407
Last Page:1414
Note:
All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License http://creativecommons.org/licenses/by-nc/3.0/
Source:Brazilian Journal of Medical and Biological Research, 1997, vol.30, n.12, pp. 1407-1414 ; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200004
HeBIS-PPN:219404259
Institutes:Biowissenschaften / Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell 3.0