Inhibition of HIV-1 replication by small interfering RNAs directed against Glioma Pathogenesis Related Protein (GliPR) expression

  • Background: Previously, we showed that glioma pathogenesis related protein (GliPR) is induced in CEM T cells upon HIV-1 infection in vitro. To examine whether GliPR plays a role as HIV dependency factor (HDF), we tested the effect of GliPR suppression by siRNA on HIV-1 replication. Results: Induction of GliPR expression by HIV-1 was confirmed in P4-CCR5 cells. When GliPR was suppressed by siRNA, HIV-1 replication was significantly reduced as measured by HIV-1 transcript levels, HIV-1 p24 protein levels, and HIV-1 LTR-driven reporter gene expression, suggesting that GliPR is a cellular co-factor of HIV-1. Microarray analysis of uninfected HeLa cells following knockdown of GliPR revealed, among a multitude of gene expression alterations, a down-regulation of syndecan-1, syndecan-2, protein kinase C alpha (PRKCA), the catalytic subunit beta of cAMP-dependent protein kinase (PRKACB), nuclear receptor co-activator 3 (NCOA3), and cell surface protein CD59 (protectin), all genes having relevance for HIV-1 pathology. Conclusions: The up-regulation of GliPR by HIV-1 and the early significant inhibition of HIV-1 replication mediated by knockdown of GliPR reveal GliPR as an important HIV-1 dependency factor (HDF), which may be exploited for HIV-1 inhibition.

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Metadaten
Author:Gianni Capalbo, Thea Müller-KullerGND, Ursula Dietrich, Dieter HoelzerGND, Oliver G. OttmannORCiDGND, Urban J. Scheuring
URN:urn:nbn:de:hebis:30-76740
DOI:https://doi.org/10.1186/1742-4690-7-26
ISSN:1742-4690
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/20356381
Parent Title (English):Retrovirology
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Language:English
Date of Publication (online):2010/04/27
Date of first Publication:2010/03/31
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2010/04/27
Volume:7
Issue:Art. 26
Page Number:10
First Page:1
Last Page:10
Note:
© 2010 Capalbo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source:Retrovirology 2010, 7:26 ; doi:10.1186/1742-4690-7-26 ; http://www.retrovirology.com/content/7/1/26
HeBIS-PPN:223104078
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 2.0