Bone substitute effect on vascularization and bone remodeling after application of phVEGF165 transfected BMSC

  • VEGF (vascular endothelial growth factor) promotes vascularization and remodeling of bone substitutes. The aim of this study was to examine the effect of distinct resorbable ceramic carriers on bone forming capacities of VEGF transfected bone marrow stromal cells (BMSC). A critical size defect of the radius in rabbits was filled either by a low surface scaffold called beta-TCP (tricalciumphsphate) or the high surface scaffold CDHA (calcium deficient hydroxy-apatite) loaded with autologous BMSC, which were either transfected with a control plasmid or a plasmid coding for phVEGF165. They were compared to unloaded scaffolds. Thus, six treatment groups (n = 6 in each group) were followed by X-ray over 16 weeks. After probe retrieval, the volume of new bone was measured by micro-CT scans and vascularization was assessed in histology. While only minor bone formation was found in both carriers when implanted alone, BMSC led to increased osteogenesis in both carriers. VEGF promoted vascularization of the scaffolds significantly in contrast to BMSC alone. Bone formation was increased in the beta-TCP group, whereas it was inhibited in the CDHA group that showed faster scaffold degradation. The results indicate that the interaction of VEGF transfected BMSC with resorbable ceramic carrier influences the ability to promote bone healing.

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Metadaten
Author:Florian Geiger, Mirjam Beverungen, Helga Lorenz, Julia Wieland, Michael Fehr, Philip Kasten
URN:urn:nbn:de:hebis:30:3-252101
DOI:https://doi.org/10.3390/jfb3020313
ISSN:2079-4983
Parent Title (English):Journal of functional biomaterials
Publisher:Molecular Diversity Preservation International (MDPI)
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2012/04/23
Date of first Publication:2012/04/19
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2012/06/22
Tag:BMSC; VEGF; angiogenesis; bone substitutes; osteogenesis
Volume:3
Issue:2
Page Number:14
First Page:313
Last Page:326
Note:
© 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
HeBIS-PPN:357456467
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 3.0