Novel strategies to improve co-fermentation of pentoses with D-glucose by recombinant yeast strains in lignocellulosic hydrolysates

  • Economically feasible production of second-generation biofuels requires efficient co-fermentation of pentose and hexose sugars in lignocellulosic hydrolysates under very harsh conditions. Baker’s yeast is an excellent, traditionally used ethanol producer but is naturally not able to utilize pentoses. This is due to the lack of pentose-specific transporter proteins and enzymatic reactions. Thus, natural yeast strains must be modified by genetic engineering. Although the construction of various recombinant yeast strains able to ferment pentose sugars has been described during the last two decades, their rates of pentose utilization is still significantly lower than D-glucose fermentation. Moreover, pentoses are only fermented after D-glucose is exhausted, resulting in an uneconomical increase in the fermentation time. In this addendum, we discuss novel approaches to improve utilization of pentoses by development of specific transporters and substrate channeling in enzyme cascades. Addendum to: T Subtil, E Boles. Competition between pentoses and glucose during uptake and catabolism in recombinant Saccharomyces cerevisiae. Biotechnol Biofuels 2012; 5: 14 PMID: 22424089 DOI: 10.1186/1754-6834-5-14

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Metadaten
Author:Igor-Mislav OrebORCiDGND, Heiko Dietz, Alexander Farwick, Eckhard BolesORCiD
URN:urn:nbn:de:hebis:30:3-288612
DOI:https://doi.org/10.4161/bioe.21444
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/22892590
Parent Title (English):Bioengineered
Publisher:Landes Bioscience
Place of publication:Austin, Tex.
Document Type:Article
Language:English
Year of Completion:2012
Year of first Publication:2012
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2013/02/14
Tag:bioethanol; co-fermentation of pentoses with glucose; substrate channeling; sugar transport
Volume:3
Issue:6
Page Number:5
First Page:347
Last Page:351
Note:
Copyright © 2012 Landes Bioscience. This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
HeBIS-PPN:331489473
Institutes:Biowissenschaften / Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell 3.0