Comparative analysis of single and combined APP/APLP knockouts reveals reduced spine density in APP-KO mice that is prevented by APPα expression

  • Synaptic dysfunction and synapse loss are key features of Alzheimer's pathogenesis. Previously, we showed an essential function of APP and APLP2 for synaptic plasticity, learning and memory. Here, we used organotypic hippocampal cultures to investigate the specific role(s) of APP family members and their fragments for dendritic complexity and spine formation of principal neurons within the hippocampus. Whereas CA1 neurons from APLP1-KO or APLP2-KO mice showed normal neuronal morphology and spine density, APP-KO mice revealed a highly reduced dendritic complexity in mid-apical dendrites. Despite unaltered morphology of APLP2-KO neurons, combined APP/APLP2-DKO mutants showed an additional branching defect in proximal apical dendrites, indicating redundancy and a combined function of APP and APLP2 for dendritic architecture. Remarkably, APP-KO neurons showed a pronounced decrease in spine density and reductions in the number of mushroom spines. No further decrease in spine density, however, was detectable in APP/APLP2-DKO mice. Mechanistically, using APPsalpha-KI mice lacking transmembrane APP and expressing solely the secreted APPsalpha fragment we demonstrate that APPsalpha expression alone is sufficient to prevent the defects in spine density observed in APP-KO mice. Collectively, these studies reveal a combined role of APP and APLP2 for dendritic architecture and a unique function of secreted APPs for spine density.

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  • Additional file 1: Figure 1, Figure2, Figure 3: Comparative analysis of single and combined APP/APLP knockouts reveals reduced spine density in APP-KO mice that is prevented by APPsα expression.

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Author:Sascha W. Weyer, Marta Zagrebelsky, Ulrike Herrmann, Meike Hick, Lennard Ganss, Julia Gobbert, Morna Gruber, Christine Altmann, Martin Korte, Thomas Deller, Ulrike C. Müller
URN:urn:nbn:de:hebis:30:3-334027
DOI:https://doi.org/10.1186/2051-5960-2-36
ISSN:2051-5960
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/24684730
Parent Title (English):Acta Neuropathologica Communications
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Language:English
Year of Completion:2014
Date of first Publication:2014/03/31
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2014/04/08
Tag:Alzheimer; Amyloid precursor protein; Knockout; Neuronal morphology; Spine density
Volume:2
Issue:Art. 36
Page Number:15
First Page:1
Last Page:15
Note:
© 2014 Weyer et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
HeBIS-PPN:364444150
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0