Combined transcript, proteome, and metabolite analysis of transgenic maize seeds engineered for enhanced carotenoid synthesis reveals pleotropic effects in core metabolism

  • The aim of this study was to assess whether endosperm-specific carotenoid biosynthesis influenced core metabolic processes in maize embryo and endosperm and how global seed metabolism adapted to this expanded biosynthetic capacity. Although enhancement of carotenoid biosynthesis was targeted to the endosperm of maize kernels, a concurrent up-regulation of sterol and fatty acid biosynthesis in the embryo was measured. Targeted terpenoid analysis, and non-targeted metabolomic, proteomic, and transcriptomic profiling revealed changes especially in carbohydrate metabolism in the transgenic line. In-depth analysis of the data, including changes of metabolite pools and increased enzyme and transcript concentrations, gave a first insight into the metabolic variation precipitated by the higher up-stream metabolite demand by the extended biosynthesis capacities for terpenoids and fatty acids. An integrative model is put forward to explain the metabolic regulation for the increased provision of terpenoid and fatty acid precursors, particularly glyceraldehyde 3-phosphate and pyruvate or acetyl-CoA from imported fructose and glucose. The model was supported by higher activities of fructokinase, glucose 6-phosphate isomerase, and fructose 1,6-bisphosphate aldolase indicating a higher flux through the glycolytic pathway. Although pyruvate and acetyl-CoA utilization was higher in the engineered line, pyruvate kinase activity was lower. A sufficient provision of both metabolites may be supported by a by-pass in a reaction sequence involving phosphoenolpyruvate carboxylase, malate dehydrogenase, and malic enzyme.

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Author:Mathilde Decourcelle, Laura Perez-Fons, Sylvain Baulande, Sabine Steiger, Linhdavanh Couvelard, Sonia Hem, Changfu Zhu, Teresa Capell, Paul Christou, Paul J. Fraser, Gerhard SandmannORCiD
URN:urn:nbn:de:hebis:30:3-372250
DOI:https://doi.org/10.1093/jxb/erv120
ISSN:1460-2431
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/25796085
Parent Title (English):The journal of experimental botany
Publisher:Univ. Press
Place of publication:Oxford
Document Type:Article
Language:English
Date of Publication (online):2015/03/20
Date of first Publication:2015/03/20
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2015/04/20
Tag:GM maize; Genetically engineered carotenoid biosynthesis; metabolomics; pathway regulation; proteomics; transcriptomics
Volume:66
Issue:11
Page Number:10
First Page:3141
Last Page:3150
Note:
© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
HeBIS-PPN:368987507
Institutes:Biowissenschaften / Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik)
Sammlungen:Universitätspublikationen
Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 3.0