Scanning acoustic microscopy : a novel noninvasive method to determine tumor interstitial fluid pressure in a xenograft tumor model

  • Elevated tumor interstitial fluid pressure (TIFP) is a prominent feature of solid tumors and hampers the transmigration of therapeutic macromolecules, for example, large monoclonal antibodies, from tumor-supplying vessels into the tumor interstitium. TIFP values of up to 40 mm Hg have been measured in experimental solid tumors using two conventional invasive techniques: the wick-in-needle and the micropuncture technique. We propose a novel noninvasive method of determining TIFP via ultrasonic investigation with scanning acoustic microscopy at 30-MHz frequency. In our experimental setup, we observed for the impedance fluctuations in the outer tumor hull of A431-vulva carcinoma–derived tumor xenograft mice. The gain dependence of signal strength was quantified, and the relaxation of tissue was calibrated with simultaneous hydrostatic pressure measurements. Signal patterns from the acoustical images were translated into TIFP curves, and a putative saturation effect was found for tumor pressures larger than 3 mm Hg. This is the first noninvasive approach to determine TIFP values in tumors. This technique can provide a potentially promising noninvasive assessment of TIFP and, therefore, can be used to determine the TIFP before treatment approach as well to measure therapeutic efficacy highlighted by lowered TFP values.
Author:Matthias HofmannGND, Ralph PflanzerGND, Anowarul Habib, Amit Shelke, Jürgen Bereiter-HahnORCiDGND, August BerndORCiDGND, Roland KaufmannGND, Robert Alexander SaderORCiDGND, Stefan KippenbergerORCiDGND
Pubmed Id:
Parent Title (German):Translational oncology
Publisher:[s. n.]
Place of publication:Ann Arbor, Mich.
Document Type:Article
Date of Publication (online):2016/11/28
Date of first Publication:2016/04/21
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2016/11/28
Page Number:5
First Page:179
Last Page:183
© 2016 The Authors. Published by Elsevier Inc. on behalf of Neoplasia Press, Inc. This is an open access article under the CC BY-NC-ND license (
Institutes:Biowissenschaften / Biowissenschaften
Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0