Stability of BDNF in human samples stored up to 6 months and correlations of serum and EDTA-plasma concentrations

  • Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at −80 °C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to rs = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations.

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Author:Maryna Polyakova, Haiko Schlögl, Julia Sacher, Maren Schmidt-KassowORCiDGND, Jochen KaiserORCiDGND, Michael Stumvoll, Jürgen Kratzsch, Matthias Schroeter
Pubmed Id:
Parent Title (English):International journal of molecular sciences
Publisher:Molecular Diversity Preservation International
Place of publication:Basel
Contributor(s):Guiting Lin
Document Type:Article
Year of Completion:2017
Date of first Publication:2017/06/03
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/01/02
Tag:BDNF; EDTA-plasma; acceptable change limit; brain-derived neurotrophic factor; inter-assay precision; serum; stability
Issue:6, Art. 1189
Page Number:11
First Page:1
Last Page:11
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0