The benzodiazepine-like natural product tilivalline is produced by the entomopathogenic bacterium Xenorhabdus eapokensis
- The pyrrolobenzodiazepine tilivalline (1) was originally identified in the human gut pathobiont Klebsiella oxytoca, the causative agent of antibiotic-associated hemorrhagic colitis. Here we show the identification of tilivalline and analogs thereof in the entomopathogenic bacterium Xenorhabdus eapokensis as well as the identification of its biosynthesis gene cluster encoding a bimodular non-ribosomal peptide synthetase. Heterologous expression of both genes in E. coli resulted in the production of 1 and from mutasynthesis and precursor directed biosynthesis 11 new tilivalline analogs were identified in X. eapokensis. These results allowed the prediction of the tilivalline biosynthesis being similar to that in K. oxytoca.
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| Author: | Hendrik WolffGND, Helge Björn BodeORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-458804 |
| DOI: | https://doi.org/10.1371/journal.pone.0194297 |
| ISSN: | 1932-6203 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/29596433 |
| Parent Title (English): | PLoS one |
| Publisher: | PLoS |
| Place of publication: | Lawrence, Kan. |
| Contributor(s): | Willem van Berkel |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2018 |
| Date of first Publication: | 2018/03/29 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2018/04/03 |
| Tag: | Biosynthesis; Indoles; Insects; Klebsiella oxytoca; Network analysis; Operons; Proline; Protein domains |
| Volume: | 13 |
| Issue: | (3): e0194297 |
| Page Number: | 19 |
| First Page: | 1 |
| Last Page: | 19 |
| Note: | Copyright: © 2018 Wolff, Bode. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| HeBIS-PPN: | 432100733 |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Open-Access-Publikationsfonds: | Biowissenschaften |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
