Endogenous AJAP1 associates with the cytoskeleton and attenuates angiogenesis in endothelial cells

  • The adherens junction associated protein 1 (AJAP1, aka shrew-1) is presumably a type-I transmembrane protein localizing and interacting with the E-cadherin-catenin complex. In various tumors, AJAP1 expression is reduced or lost, including hepatocellular and esophageal squamous cell carcinoma, and glial-derived tumors. The aberrant expression of AJAP1 is associated with alterations in cell migration, invasion, increased tumor growth, and tumor vascularization, suggesting AJAP1 as a putative tumor suppressor. We show that AJAP1 attenuates sprouting angiogenesis by reducing endothelial migration and invasion capacities. Further, we show for the first time that endogenous AJAP1 is associated with the microtubule cytoskeleton. This linkage is independent from cell confluency and stable during angiogenic sprouting in vitro. Our work suggests that AJAP1 is a putative negative regulator of angiogenesis, reducing cell migration and invasion by interfering with the microtubule network. Based on our results and those of other authors, we suggest AJAP1 as a novel tumor suppressor and diagnostic marker.

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Author:Katharina Hötte, Isabell Smyrek, Anna Starzinski-Powitz, Ernst H. K. StelzerORCiDGND
URN:urn:nbn:de:hebis:30:3-463487
DOI:https://doi.org/10.1242/bio.022335
ISSN:2046-6390
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/28483980
Parent Title (English):Biology open
Publisher:Company
Place of publication:Cambridge
Document Type:Article
Language:English
Year of Completion:2017
Date of first Publication:2017/06/15
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/04/26
Tag:AJAP1; Angiogenesis; Cell migration; Cytoskeleton; Endothelial cells; Microtubules; Shrew-1
Volume:6
Issue:6
Page Number:9
First Page:723
Last Page:731
Note:
Copyright & Usage: © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
HeBIS-PPN:431860734
Institutes:Biowissenschaften / Biowissenschaften
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Open-Access-Publikationsfonds:Biowissenschaften
Licence (German):License LogoCreative Commons - Namensnennung 3.0