Azobenzene as a photoregulator covalently attached to RNA : a quantum mechanics/molecular mechanics-surface hopping dynamics study

  • The photoregulation of nucleic acids by azobenzene photoswitches has recently attracted considerable interest in the context of emerging biotechnological applications. To understand the mechanism of photoinduced isomerisation and conformational control in these complex biological environments, we employ a Quantum Mechanics/Molecular Mechanics (QM/MM) approach in conjunction with nonadiabatic Surface Hopping (SH) dynamics. Two representative RNA–azobenzene complexes are investigated, both of which contain the azobenzene chromophore covalently attached to an RNA double strand via a β-deoxyribose linker. Due to the pronounced constraints of the local RNA environment, it is found that trans-to-cis isomerization is slowed down to a time scale of ∼10–15 picoseconds, in contrast to 500 femtoseconds in vacuo, with a quantum yield reduced by a factor of two. By contrast, cis-to-trans isomerization remains in a sub-picosecond regime. A volume-conserving isomerization mechanism is found, similarly to the pedal-like mechanism previously identified for azobenzene in solution phase. Strikingly, the chiral RNA environment induces opposite right-handed and left-handed helicities of the ground-state cis-azobenzene chromophore in the two RNA–azobenzene complexes, along with an almost completely chirality conserving photochemical pathway for these helical enantiomers.

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Author:Padmabati Mondal, Giovanni Granucci, Dominique Rastädter, Maurizio Persico, Irene Burghardt
URN:urn:nbn:de:hebis:30:3-468248
DOI:https://doi.org/10.1039/c8sc00072g
ISSN:2041-6539
ISSN:2041-6520
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/29899961
Parent Title (English):Chemical science
Publisher:RSC
Place of publication:Cambridge
Document Type:Article
Language:English
Year of Completion:2018
Date of first Publication:2018/04/27
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/07/03
Volume:9
Issue:20
Page Number:11
First Page:4671
Last Page:1681
Note:
This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
HeBIS-PPN:435655329
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell 3.0