Velvet domain protein VosA represses the zinc cluster transcription factor SclB regulatory network for Aspergillus nidulans asexual development, oxidative stress response and secondary metabolism

  • The NF-κB-like velvet domain protein VosA (viability of spores) binds to more than 1,500 promoter sequences in the filamentous fungus Aspergillus nidulans. VosA inhibits premature induction of the developmental activator gene brlA, which promotes asexual spore formation in response to environmental cues as light. VosA represses a novel genetic network controlled by the sclB gene. Bfunction is antagonistic to VosA, because it induces the expression of early activator genes of asexual differentiation as flbC and flbD as well as brlA. The SclB controlled network promotes asexual development and spore viability, but is independent of the fungal light control. SclB interactions with the RcoA transcriptional repressor subunit suggest additional inhibitory functions on transcription. SclB links asexual spore formation to the synthesis of secondary metabolites including emericellamides, austinol as well as dehydroaustinol and activates the oxidative stress response of the fungus. The fungal VosA-SclB regulatory system of transcription includes a VosA control of the sclB promoter, common and opposite VosA and SclB control functions of fungal development and several additional regulatory genes. The relationship between VosA and SclB illustrates the presence of a convoluted surveillance apparatus of transcriptional control, which is required for accurate fungal development and the linkage to the appropriate secondary metabolism.
Author:Karl G. Thieme, Jennifer Gerke, Christoph Sasse, Oliver Valerius, Sabine Thieme, Razieh Karimi, Antje Katharina Heinrich, Florian Finkernagel, Kristina Smith, Helge Björn BodeORCiDGND, Michael Freitag, Arthur F. J. Ram, Gerhard H. Braus
Pubmed Id:
Parent Title (English):PLoS Genetics
Publisher:Public Library of Science
Place of publication:San Francisco, Calif.
Contributor(s):Gregory P. Copenhaver
Document Type:Article
Year of Completion:2018
Date of first Publication:2018/07/25
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/08/14
Tag:Aspergillus nidulans; Fungal genetics; Fungal spores; Gene expression; Gene regulation; Oxidative stress; Secondary metabolism; Transcription factors
Issue:(7): e1007511
Page Number:45
First Page:1
Last Page:45
Copyright: © 2018 Thieme et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Correction erschienen in: PLoS Genetics, volume 14, issue 8, e1007638 (2018), doi:10.1371/journal.pgen.1007638
Institutes:Biowissenschaften / Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0