Comparative proteomics reveals a diagnostic signature for pulmonary head‐and‐neck cancer metastasis

  • Patients with head‐and‐neck cancer can develop both lung metastasis and primary lung cancer during the course of their disease. Despite the clinical importance of discrimination, reliable diagnostic biomarkers are still lacking. Here, we have characterised a cohort of squamous cell lung (SQCLC) and head‐and‐neck (HNSCC) carcinomas by quantitative proteomics. In a training cohort, we quantified 4,957 proteins in 44 SQCLC and 30 HNSCC tumours. A total of 518 proteins were found to be differentially expressed between SQCLC and HNSCC, and some of these were identified as genetic dependencies in either of the two tumour types. Using supervised machine learning, we inferred a proteomic signature for the classification of squamous cell carcinomas as either SQCLC or HNSCC, with diagnostic accuracies of 90.5% and 86.8% in cross‐ and independent validations, respectively. Furthermore, application of this signature to a cohort of pulmonary squamous cell carcinomas of unknown origin leads to a significant prognostic separation. This study not only provides a diagnostic proteomic signature for classification of secondary lung tumours in HNSCC patients, but also represents a proteomic resource for HNSCC and SQCLC.
Author:Hanibal Bohnenberger, Lars Kaderali, Philipp Ströbel, Diego Yepes, Uwe Plessmann, Neekesh V. Dharia, Sha Yao, Carina Heydt, Sabine Merkelbach-Bruse, Alexander Emmert, Jonatan Hoffmann, Julius Bodemeyer, Kirsten Reuter-Jessen, Anna-Maria Lois, Leif Hendrik Dröge, Philipp Baumeister, Christoph Walz, Lorenz Biggemann, Roland Walter, Björn Häupl, Federico Comoglio, Kuan-Ting Pan, Sebastian Scheich, Christof Lenz, Stefan Küffer, Felix Bremmer, Julia Kitz, Maren Sitte, Tim Beißbarth, Marc Hinterthaner, Martin Sebastian, Joachim Lotz, Joachim Lotz, Hendrik Wolff, Bernhard Christoph Danner, Christian Hubertus BrandtsORCiDGND, Reinhard Büttner, Martin Canis, Kimberly Stegmaier, Hubert ServeORCiDGND, Henning Urlaub, Thomas OellerichORCiD
Pubmed Id:
Parent Title (English):EMBO molecular medicine
Publisher:Wiley-VCH ; MBO Press
Place of publication:Weinheim ; Heidelberg
Document Type:Article
Year of Completion:2018
Date of first Publication:2018/08/10
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/11/01
Tag:Biomarker; head-and-neck cancer; lung cancer; metastasis; proteomics
Issue:9, e8428
Page Number:15
First Page:1
Last Page:15
© 2018 The Authors. Published under the terms of the CC BY 4.0 license
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0