A superfolder variant of pH-sensitive pHluorin for in vivo pH measurements in the endoplasmic reticulum
- Many cellular processes are regulated via pH, and maintaining the pH of different organelles is crucial for cell survival. A pH-sensitive GFP variant, the so-called pHluorin, has proven to be a valuable tool to study the pH of the cytosol, mitochondria and other organelles in vivo. We found that the fluorescence intensity of Endoplasmic Reticulum (ER)-targeted pHluorin in the yeast Saccharomyces cerevisiae was very low and barely showed pH sensitivity, probably due to misfolding in the oxidative environment of the ER. We therefore developed a superfolder variant of pHluorin which enabled us to monitor pH changes in the ER and the cytosol of S. cerevisiae in vivo. The superfolder pHluorin variant is likely to be functional in cells of different organisms as well as in additional compartments that originate from the secretory pathway like the Golgi apparatus and pre-vacuolar compartments, and therefore has a broad range of possible future applications.
Download full text files
Additional Services
| Author: | Mara Reifenrath, Eckhard BolesORCiD |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-479261 |
| DOI: | https://doi.org/10.1038/s41598-018-30367-z |
| ISSN: | 2045-2322 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/30097598 |
| Parent Title (English): | Scientific reports |
| Publisher: | Macmillan Publishers Limited, part of Springer Nature |
| Place of publication: | [London] |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2018 |
| Date of first Publication: | 2018/08/10 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2018/10/30 |
| Tag: | Endoplasmic reticulum; Fluorescent proteins; Golgi |
| Volume: | 8 |
| Issue: | 1, Art. 11985 |
| Page Number: | 8 |
| First Page: | 1 |
| Last Page: | 8 |
| Note: | Rights and permissions: Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| HeBIS-PPN: | 439887917 |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Open-Access-Publikationsfonds: | Biowissenschaften |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
