Cryo-EM structure of respiratory complex I at work

  • Mitochondrial complex I has a key role in cellular energy metabolism, generating a major portion of the proton motive force that drives aerobic ATP synthesis. The hydrophilic arm of the L-shaped ~1 MDa membrane protein complex transfers electrons from NADH to ubiquinone, providing the energy to drive proton pumping at distant sites in the membrane arm. The critical steps of energy conversion are associated with the redox chemistry of ubiquinone. We report the cryo-EM structure of complete mitochondrial complex I from the aerobic yeast Yarrowia lipolytica both in the deactive form and after capturing the enzyme during steady-state activity. The site of ubiquinone binding observed during turnover supports a two-state stabilization change mechanism for complex I.
Author:Kristian Kurt Parey, Ulrich BrandtORCiDGND, Hao Xie, Deryck J. Mills, Karin Siegmund, Janet Vonck, Werner Kühlbrandt, Volker Zickermann
Pubmed Id:
Parent Title (English):eLife
Publisher:eLife Sciences Publications
Place of publication:Cambridge
Contributor(s):Sjors H. W. Scheres
Document Type:Article
Year of Completion:2018
Date of first Publication:2018/10/01
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/11/29
Tag:Research article; Yarrowia lipolytica; active/deactive transition; redox-linked proton translocation; respiratory complex I; structural biology and molecular biophysics
Page Number:28
First Page:1
Last Page:28
Copyright Parey et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Institutes:Medizin / Medizin
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Angeschlossene und kooperierende Institutionen / MPI für Biophysik
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0