Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra

  • Neuropogonoid species in the lichen-forming fungal genus Usnea exhibit great morphological variation that can be misleading for delimitation of species. We specifically focused on the species delimitation of two closely-related, predominantly Antarctic species differing in the reproductive mode and representing a so-called species pair: the asexual U. antarctica and the sexual U. aurantiacoatra. Previous studies have revealed contradicting results. While multi-locus studies based on DNA sequence data provided evidence that these two taxa might be conspecific, microsatellite data suggested they represent distinct lineages. By using RADseq, we generated thousands of homologous markers to build a robust phylogeny of the two species. Furthermore, we successfully implemented these data in fine-scale population genomic analyses such as DAPC and fineRADstructure. Both Usnea species are readily delimited in phylogenetic inferences and, therefore, the hypothesis that both species are conspecific was rejected. Population genomic analyses also strongly confirmed separated genomes and, additionally, showed different levels of co-ancestry and substructure within each species. Lower co-ancestry in the asexual U. antarctica than in the sexual U. aurantiacoatra may be derived from a wider distributional range of the former species. Our results demonstrate the utility of this RADseq method in tracing population dynamics of lichens in future analyses.

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Author:Felix Grewe, Elisa Lagostina, Huini Wu, Christian Printzen, Helge Thorsten Lumbsch
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/30588165
Parent Title (English):MycoKeys
Publisher:Pensoft Publishers
Place of publication:Washington, DC [u. a.]
Contributor(s):Ekaphan Kraichak
Document Type:Article
Year of Completion:2018
Date of first Publication:2018/12/12
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2019/01/10
Tag:Antarctica; Ascomycota; Parmeliaceae; RADseq; lichens; phylogeny
Page Number:23
First Page:91
Last Page:113
Copyright Felix Grewe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Institutes:Biowissenschaften / Biowissenschaften
Angeschlossene und kooperierende Institutionen / Senckenbergische Naturforschende Gesellschaft
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik)
Licence (German):License LogoCreative Commons - Namensnennung 4.0