Reversible optical control of F1Fo‐ATP synthase using photoswitchable inhibitors

  • F1Fo‐ATP synthase is one of the best studied macromolecular machines in nature. It can be inhibited by a range of small molecules, which include the polyphenols, resveratrol and piceatannol. Here, we introduce Photoswitchable Inhibitors of ATP Synthase, termed PIAS, which were synthetically derived from these polyphenols. They can be used to reversibly control the enzymatic activity of purified yeast Yarrowia lipolyticaATP synthase by light. Our experiments indicate that the PIAS bind to the same site in the ATP synthase F1 complex as the polyphenols in their trans form, but they do not bind in their cis form. The PIAS could be useful tools for the optical precision control of ATP synthase in a variety of biochemical and biotechnological applications.
Author:Bianca Eisel, Felix Hartrampf, Thomas Meier, Dirk Trauner
Pubmed Id:
Parent Title (English):FEBS letters
Place of publication:Chichester
Contributor(s):Peter Brzezinski
Document Type:Article
Year of Completion:2018
Date of first Publication:2018/02/01
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/01/17
Tag:Yarrowia lipolytica F1Fo-ATP synthase; photopharmacology
Page Number:13
First Page:343
Last Page:355
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Angeschlossene und kooperierende Institutionen / MPI für Biophysik
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Licence (German):License LogoCreative Commons - Namensnennung 4.0