Further insights into the interaction of human and animal complement regulator factor H with viable lyme disease spirochetes

  • Spirochetes belonging to the Borrelia (B.) burgdorferi sensu lato (s.l.) complex differ in their ability to establish infection and to survive in diverse vertebrate hosts. Association with and adaption to various hosts most likely correlates with the spirochetes' ability to acquire complement regulator factor H (FH) to overcome the host's innate immune response. Here we assessed binding of serum FH from human and various animals including bovine, cat, chicken, dog, horse, mouse, rabbit, and rat to viable B. burgdorferi sensu stricto (s.s.), B. afzelii, B. garinii, B. spielmanii, B. valaisiana, and B. lusitaniae. Spirochetes ectopically producing CspA orthologs of B. burgdorferi s.s., B. afzelii, and B. spielmanii, CspZ, ErpC, and ErpP, respectively, were also investigated. Our comparative analysis using viable bacterial cells revealed a striking heterogeneity among Lyme disease spirochetes regarding their FH-binding patterns that almost mirrors the serum susceptibility of the respective borrelial genospecies. Moreover, native CspA from B. burgdorferi s.s., B. afzelii, and B. spielmanii as well as CspZ were identified as key ligands of FH from human, horse, and rat origin while ErpP appears to bind dog and mouse FH and to a lesser extent human FH. By contrast, ErpC did not bind FH from human as well as from animal origin. These findings indicate a strong restriction of distinct borrelial proteins toward binding of polymorphic FH of various vertebrate hosts.

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Author:Jovana Jasmin Mühleip, Yi-Pin LinORCiD, Peter KraiczyGND
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/30766876
Parent Title (English):Frontiers in veterinary science
Publisher:Frontiers Research Foundation
Place of publication:Lausanne
Contributor(s):Artur Summerfield
Document Type:Article
Year of Completion:2019
Date of first Publication:2019/01/31
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/02/19
Tag:Borrelia; Lyme disease; complement; factor H; hostpathogen interaction; immune evasion; innate immunity; spirochete
Issue:Art. 346
Page Number:11
First Page:1
Last Page:11
Copyright © 2019 Mühleip, Lin and Kraiczy. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0