Development of an exercise training protocol to investigate arteriogenesis in a murine model of peripheral artery disease

  • Exercise is a treatment option in peripheral artery disease (PAD) patients to improve their clinical trajectory, at least in part induced by collateral growth. The ligation of the femoral artery (FAL) in mice is an established model to induce arteriogenesis. We intended to develop an animal model to stimulate collateral growth in mice through exercise. The training intensity assessment consisted of comparing two different training regimens in C57BL/6 mice, a treadmill implementing forced exercise and a free-to-access voluntary running wheel. The mice in the latter group covered a much greater distance than the former pre- and postoperatively. C57BL/6 mice and hypercholesterolemic ApoE-deficient (ApoE-/-) mice were subjected to FAL and had either access to a running wheel or were kept in motion-restricting cages (control) and hind limb perfusion was measured pre- and postoperatively at various times. Perfusion recovery in C57BL/6 mice was similar between the groups. In contrast, ApoE-/- mice showed significant differences between training and control 7 d postoperatively with a significant increase in pericollateral macrophages while the collateral diameter did not differ between training and control groups 21 d after surgery. ApoE-/- mice with running wheel training is a suitable model to simulate exercise induced collateral growth in PAD. This experimental set-up may provide a model for investigating molecular training effects.

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Author:Ayko Bresler, Johanna Vogel, Daniel NiedererORCiDGND, Daphne Gray, Thomas Schmitz-RixenORCiDGND, Kerstin Troidl
Pubmed Id:
Parent Title (English):International journal of molecular sciences
Publisher:Molecular Diversity Preservation International
Place of publication:Basel
Document Type:Article
Year of Completion:2019
Date of first Publication:2019/08/14
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2019/08/19
Tag:arteriogenesis; exercise training; femoral artery ligation; mouse model; peripheral artery disease; running wheel; voluntary training
Issue:16, Art. 3956
Page Number:12
First Page:1
Last Page:12
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Institutes:Psychologie und Sportwissenschaften / Sportwissenschaften
Medizin / Medizin
Wissenschaftliche Zentren und koordinierte Programme / Starker Start ins Studium: Qualit├Ątspakt Lehre
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0