Identification and characterization of a thermotolerant tilling allele of heat shock binding protein 1 in tomato

  • The identification of heat stress (HS)-resilient germplasm is important to ensure food security under less favorable environmental conditions. For that, germplasm with an altered activity of factors regulating the HS response is an important genetic tool for crop improvement. Heat shock binding protein (HSBP) is one of the main negative regulators of HS response, acting as a repressor of the activity of HS transcription factors. We identified a TILLING allele of Solanum lycopersicum (tomato) HSBP1. We examined the effects of the mutation on the functionality of the protein in tomato protoplasts, and compared the thermotolerance capacity of lines carrying the wild-type and mutant alleles of HSBP1. The methionine-to-isoleucine mutation in the central heptad repeats of HSBP1 leads to a partial loss of protein function, thereby reducing the inhibitory effect on Hsf activity. Mutant seedlings show enhanced basal thermotolerance, while mature plants exhibit increased resilience in repeated HS treatments, as shown by several physiological parameters. Importantly, plants that are homozygous for the wild-type or mutant HSBP1 alleles showed no significant differences under non-stressed conditions. Altogether, these results indicate that the identified mutant HSBP1 allele can be used as a genetic tool in breeding, aiming to improve the thermotolerance of tomato varieties.

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Author:Dominik Marko, Asmaa Samir Attia El-Shershaby, Filomena Carriero, Stephan Summerer, Angelo Petrozza, Rina Iannacone, Enrico SchleiffORCiDGND, Sotirios FragkostefanakisORCiD
URN:urn:nbn:de:hebis:30:3-512683
DOI:https://doi.org/10.3390/genes10070516
ISSN:2073-4425
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/31284688
Parent Title (English):Genes
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/07/07
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2019/09/30
Tag:HSBP; Solanum lycopersicum; heat shock protein; heat stress; heat stress transcription factor; phenotyping; thermotolerance
Volume:10
Issue:7, Art. 516
Page Number:17
First Page:1
Last Page:17
Note:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
HeBIS-PPN:454035128
Institutes:Biowissenschaften / Biowissenschaften
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik)
Sammlungen:Universit├Ątspublikationen
Open-Access-Publikationsfonds:Biowissenschaften
Licence (German):License LogoCreative Commons - Namensnennung 4.0