In-depth proteome analysis highlights heparg cells as a versatile cell system surrogate for primary human hepatocytes

  • Of the hepatic cell lines developed for in vitro studies of hepatic functions as alternatives to primary human hepatocytes, many have lost major liver-like functions, but not HepaRG cells. The increasing use of the latter worldwide raises the need for establishing the reference functional status of early biobanked HepaRG cells. Using deep proteome and secretome analyses, the levels of master regulators of the hepatic phenotype and of the structural elements ensuring biliary polarity were found to be close to those in primary hepatocytes. HepaRG cells proved to be highly differentiated, with functional mitochondria, hepatokine secretion abilities, and an adequate response to insulin. Among differences between primary human hepatocytes and HepaRG cells, the factors that possibly support HepaRG transdifferentiation properties are discussed. The HepaRG cell system thus appears as a robust surrogate for primary hepatocytes, which is versatile enough to study not only xenobiotic detoxification, but also the control of hepatic energy metabolism, secretory function and disease-related mechanisms.

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Author:Georg Tascher, Audrey Burban, Sandrine Camus, Marine Plumel, Stéphanie Chanon, Rémy Le Guével, Valery Shevchenko, Alain Van Dorsselaer, Etienne Lefai, Christiane Guguen-Guillouzo, Fabrice Bertile
URN:urn:nbn:de:hebis:30:3-514682
DOI:https://doi.org/10.3390/cells8020192
ISSN:2073-4409
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/30795634
Parent Title (English):Cells
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/02/21
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/10/28
Tag:HepaRG cells; Hepatocytes; detoxification; hepatic phenotype; liver cell lines; liver diseases; liver metabolism; proteome; secretome; transdifferentiation
Volume:8
Issue:2, Art. 192
Page Number:25
First Page:1
Last Page:25
Note:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
HeBIS-PPN:455331669
Institutes:Medizin / Medizin
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0