Flotillins regulate focal adhesions by interacting with α-actinin and by influencing the activation of focal adhesion kinase

  • Cell–matrix adhesion and cell migration are physiologically important processes that also play a major role in cancer spreading. In cultured cells, matrix adhesion depends on integrin-containing contacts such as focal adhesions. Flotillin-1 and flotillin-2 are frequently overexpressed in cancers and are associated with poor survival. Our previous studies have revealed a role for flotillin-2 in cell–matrix adhesion and in the regulation of the actin cytoskeleton. We here show that flotillins are important for cell migration in a wound healing assay and influence the morphology and dynamics of focal adhesions. Furthermore, anchorage-independent growth in soft agar is enhanced by flotillins. In the absence of flotillins, especially flotillin-2, phosphorylation of focal adhesion kinase and extracellularly regulated kinase is diminished. Flotillins interact with α-actinin, a major regulator of focal adhesion dynamics. These findings are important for understanding the molecular mechanisms of how flotillin overexpression in cancers may affect cell migration and, especially, enhance metastasis formation.

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Author:Antje Banning, Tanja Babuke, Nina Susanne KurrleORCiDGND, Melanie Meister, Mika O. Ruonala, Ritva Tikkanen
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/29642469
Parent Title (English):Cells
Place of publication:Basel
Document Type:Article
Year of Completion:2018
Date of first Publication:2018/04/07
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/10/24
Tag:cancer; cell migration; focal adhesion; metastasis
Page Number:20
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Institutes:Biowissenschaften / Biowissenschaften
Medizin / Medizin
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0